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1	Biology	ABS-30
Effect of Sea Surface Temperature on The Metal Zn Contents in Inshore and Offshore Water of Blanakan Coast, West Java, Indonesia Noverita Dian Takarina* Department of Biology, Faculty of Mathematics and Natural Science, University of Indonesia, Depok Abstract The Sea Surface Temperature (SST) is known as one of prominent factors affecting the concentration of metals in the marine environment. Hence, this research is aiming to study the effect of SST on Zn content distribution in the seawater of Blanakan coast. The Zn was measured from sampled seawater collected from 7 stations. Those stations were representing inshore and offshore of Blanakan coast. The sampled water then analyzed by AAS to obtain the Zn contents. The result confirms a positive effect of SST on Zn contents (r2 = 0.18). An increase of SST from 25 to 31 0C was followed by increase of Zn in seawater from 0.31 to 0.50 mg/l of water. Respectively, all Zn values in Blanakan coast have exceeded the permissible limit of EPA (0.09 mg/l for seawater). The effect of SST on the Zn contents is observed in the offshore rather than inshore water of Blanakan coast. This research also presents a 12 month Zn prediction. Following the SST fluctuation, it is predicted that Zn in seawater will be higher in February-May and lower in July-September. Keywords: Coast; Metal; Offshore; Sea Surface Temperature; Inshore PermaLink | Plain Format | Corresponding Author (Noverita Dian Takarina)

2	Biology	ABS-293
GC-MS Analysis of Bioactive Compounds on Ethanolic Thelenota ananas from Selayar Island A. Munisa (a) , Yusminah Hala (a) , A. Muflihunna (b) a) Departement od Biology, Universitas Negeri Makassar, Makassar, b) Faculty of pharmacy, Universitas Muslim Indonesia, Makassar, Abstract The present investigation was carried out to characterize the bioactive compounds present in extract of Thelenota ananas using Gas Chromatography-Mass Spectrum (GC-MS). The results of the GC-MS analysis provide different peaks determining the presence of 10 phytochemical compounds with different therapeutic activities. The major hexadecanoic acid, ethyl ester (55.47), [1,1'-bibicyclo[2.2.2]octane]-4-carboxylic acid (21.07), and eicosane 4.72) and minor compounds were also present. From the results, it could be concluded that Thelenota ananas contains various bioactive compounds have various biological activities. Therefore, it is recommended as a sea cucumber of phytopharmaceutical importance. Keywords: GC-MS, Thelenota ananas, sea cucumber, Selayar Island PermaLink | Plain Format | Corresponding Author (Andi Mu’nisa)

3	Biology	ABS-56
POPULATION ESTIMATION AND HABITAT CHARACTERISTICS Rhacophorus reinwardtii IN THE SECONDARY FOREST RANCA UPAS CIWIDEY Hernawati, Tina Safaria Nilawati, Kemas Muhammad Abiyyu Ilham Malik Departement of Biology Education Universitas Pendidikan Indonesia Bandung Abstract Amphibians can be used as environmental bioindicators or health measures of an environment because they are sensitive to changes in environmental factors. Some of the main habitat of amphibians are primary and secondary forests, swamps, rivers, lakes and ponds. One of the endangered amphibians were Rhacophorus reinwardtii. The secondary forest in Ranca Upas Ciwidey was one of the habitat where Rhacophorus reinwardtii can be found. There are many significant changes to this region due to the expansion of the campground and tourist areas, thus affecting the population of Rhacophorus reinwardtii. This study aims to describe the habitat characteristics and population estimation of Rhacophorus reinwardtii in the secondary forest Ranca Upas. Habitat characteristics were analyzed descriptively. Habitat and population data are collected in two locations near rivers and stagnant water. The research area has measured 522m2 and width of 528m2. Capture-Mark-Release-Recapture Method was used in each region to collect population data. Population abundance were calculated using the Schnabel formula, population distribution were estimated using the Morista index. The caught frogs were marked using a modified shoelace. Habitat Rhacophorus reinwardtii was of river and stagnant water with an altitude of 1630-1680m asl, pH of water neutral and acidic, average velocity of the river was 0m/s and 0.04m/s, the water substrates were sand and mud, with average temperature air 16.7ºC and 17ºC, average water temperature of 14.7ºC, average humidity of air 94%. The type of plant that is often the habitat of Rhacophorus reinwardtii was Brugmansia sp. and Cyathea sp. The population estimation of the two observation areas were 45 individuals and 30 individuals with a density of 9 individuals/100m2 and 6 individuals/100m2. It can be concluded that the population distribution of Rhacophorus reinwardtii in both habitats clustered. Keywords: Keywords: Rhacophorus reinwardtii, habitat, abundance, density, distribution. PermaLink | Plain Format | Corresponding Author (Hernawati Hernawati)

4	Biology	ABS-313
ANTIBACTERIAL ACTIVITY OF Lannea coromandelica EXTRACT Hartati, Syamsuddin, Hilda Karim, Halifah Pagarra Biology of Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar Abstract This research that aims for antibacterial activity Lannea coromandelica extract. The klika was extracted by wet and dry method using ethyl acetate and ethanol 70% that acquired 4 types of extract they are ethanol extract 70% of wet klika Kayu Jawa (KJBE70%), extract ethanol 70% of dry klika Kayu Jawa (KJKE70%), ethyl acetate extract of dry klika Kayu Jawa (KJKEA), and extract of wet klika Kayu Jawa (KJBEA). Extracted by maceration, dan then antibacterial acivity. The results from this research shown that all extracts have antibacterial activity to inhibited bacteria test growth (Escherichia coli, Staphylococcus aureus, Pseudomonas sp., and Vibrio parahaemolyticus. Keywords: Lannea coromandelica, antibacterial activity PermaLink | Plain Format | Corresponding Author (Hartati Hartati)

5	Biology	ABS-314
Need Analysis for Communities Around the Mallawa Resort of Bantimurung Bulusaraung National Park to Conservation of Natural Orchids St. Fatmah Hiola (a) and Gufran Darma Dirawan (b) a) Biology Department, Universitas Negeri Makassar b) Environmental Education, Universitas Negeri Makassar Abstract Natural orchids or wild orchids are often used as the main ingredient for obtaining commercial types of hybrids, but the existence of these wild orchid species is often threatened with extinction due to narrow land, because many are used for settlements, plantations or due to natural damage. In addition, the taking of orchids in nature without considering its sustainability. The purpose of this study was to explore information about community exceptions around the study area for the preservation of natural orchids. The subjects in this study were the community around Mallawa Resort, Bantimurung Bulusaraung National Park. This type of research is descriptive using survey methods by conducting interviews and distributing questionnaires to explore information about the needs of the community related to the conservation of natural orchids. The results revealed that basically the community around the Mallawa Resort area was familiar with natural orchid plants (65.93%) but knowledge of natural orchids and their preservation was still lacking (38.27). Generally, people have a caring attitude towards the existence of natural orchids (68.52%). This can be seen from the orchids that are owned by the community generally comes from nature (50.62%) and have been able to develop them (46.91%). However, only a few people feel the benefits of developing natural orchids (22.84%), and involvement in the conservation of natural orchids carried out by the government and other parties (11-38%). The conclusion of this study illustrates the need for an activity to preserve natural orchids through the development of a training model for the community around the study area. Keywords: Conservation, Mallawa Resort, Natural orchids, Need analysis of community PermaLink | Plain Format | Corresponding Author (St. Fatmah Hiola)

6	Biology	ABS-316
PRODUCTION OF WHITE LEG SHRIMP, Litopenaus vannamei BY APPLICATION OF BIOFLOC TECHNOLOGY ON INTENSIVE SHRIMP GROWOUT PONDS Muhammad Junda, Halifah Pagarra, Surachman Nur Dept of Biological Science, Faculty of Mathematics and Natural Sciences, Makassar State University Abstract The objective of this study is to investigate the production of white leg shrimp, L vannamei by application of biofloc technology on intensive shrimp grow out ponds. This study was carried out on five of shrimp grow out ponds with different pond size and zero water exchange management strategy. These shrimps were reared with intensive systems such as shrimp seed post larvae (PL) 10, commercial feed, aeration with paddle wheels, mineral, vitamin and molasses as well as floc-forming microbes and natural herb fermentation. Shrimp grow out ponds with condition as follows : water depth, 90 – 120 cm, feeding rate four times a day, aeration 24 hours in cultured period. Water quality indicators were measured ever day such as : survival rate, average daily gain, mean body weight, and feed conversion ratio. The results of this study showed that shrimp yield were 1,250 – 6,300 kg, growth rate such as : ADG, 0,32 – 0,40; survival rate 85 – 95 %; MBW 20, 23 – 27.30 and FCR = 1.10 – 1.20. Water quality parameters : pH = 7,2 – 9.5; salinity : 15 – 25 ppt ; temperature : 28 – 31 0 C; water depth : 90 – 120; tranparancy : 25 – 40; dissolved oxygen : 3.0 – 5.2; . In conclution, Application of biofloc technology with zero water exchange increased shrimp production significantly and water quality parameters were in range suitable for shrimp cultured growth. Keywords: application biofloc technology, intensive production, Litopenaeus vannamei, zero water exchange. PermaLink | Plain Format | Corresponding Author (Muhammad Junda)

7	Biology	ABS-61
Biomonitoring of Cilaja River based on benthos community, in Ujung Berung Bandung within 2013-2017 Hertien Koosbandiah Surtikanti Departemen Pendidikan Biologi Program Studi Biologi, FPMIPA UPI Abstract Water catchment area of Cilaja is located in along Giri Mekar and Sindanglaya village, Ujung Berung, Bandung. This river has high values for the people living surrounding that area. It supports clean water supply, coffee plantation, agriculture area and fish farming area. Field study is done to monitor water quality due to high variation of human activities. Three location sites (coffee/pinus plantation, agriculture and residential area) were selected. Bioindicator benthos were collected from these site locations. Chemical-physical water were measured and analysed. This biomonitoring study is done between year 2013-2017. The results show that based on the distribution and composition of bioindicator benthos, the three differrent land uses has good water quality within 5 years. People attitudes in environmental aware play an important role in conserving water quality for long period of time. Keywords: water quality, bioindicator benthos, human activities, chemical water PermaLink | Plain Format | Corresponding Author (Hertien Koosbandiah Surtikanti)

8	Biology	ABS-322
Capability of Ludwigia adcendens and Ludwigia grandiflora as Cd (cadmium) Phytoremediator Fida Rachmadiarti, Marátus Sholikah Universitas Negeri Surabaya Abstract Ludwigia adcendens and Ludwigia grandiflora are water weeds rapidly grow and disperse. This plant is mainly found in ponds, swamps, and polluted water channels. This means that both plants are capable of absorbing organic and inorganic waste, including heavy metals as Cd (cadmium). The objective of the research to describe capability of L. grandiflora and L. adcendens in Cd Solution at various concentration as individual species or as consortium. These plants were acclimatized first in hydroponic for 14 days, then moved to medium with varying treatments; only L. grandiflora, only L. adcendens and combination of both species at different Cd concentration (0, 5, 10, and 15 ppm). Level of Cd was analyzed at 7th and 14th day using Atomic Absorption Spectrophotometer (AAS), while wet biomass of each group was compared. Data collected was analyzed statistically. Capability of L. grandiflora and L. adcendens in Cd Solution from low to high was found to be L. grandiflora, L. adcendens > consortium of both species, in which bioconcentration factor (BCF) > 1 and translocation factor (TF) < 1. Concentration of Cd was also found to be higher in the roots than in leaves. Colour of leaves also supported these plants as Cd phytoremediator; plants with higher Cd accumulation had yellow leaves compared to plants accumulated lower Cd level. Keywords: Ludwigia adcendens, Ludwigia grandiflora, BCF, TF, and Cd metal PermaLink | Plain Format | Corresponding Author (Fida Rachmadiarti)

9	Biology	ABS-330
Potential of Secondary Metabolite Cromolaena odorata as Antibacterial Againts Pathogenic Bacteria Endang Sulistyarini Gultom, Hasruddin, Meida Nugrahalia Universitas Negeri Medan Abstract The purpose of this study was to isolate secondary metabolites from the ethanol extract of chirinyuh leaves (Cromolaena odorata) which have the potential to be anti-bacterial. The results of this study are expected to get an additional inventory of medicinal plants and in the long run add to the economic value of marketable herbal medicines. The planned method is to obtain extract by maceration of Harborn (1987) method, then the extract activity test which is highest in antibacterial was carried out. To test the antibacterial activity, a diffusion method is used. Furthermore, the extract which has the highest antibacterial activity was identified to be continued to the next stage. Based on the results obtained, the ethanol extract of the kirinyuh leaf contains secondary metabolites of flavonoids, alkaloids, tannins and saponins which have antibacterial activity. This was obtained from the ability of the extract to inhibit the growth of Staphylococcus aureus, Eschericihia coli, and Pseudomonas aeruginosa test bacteria with inhibitory zones of 12 mm, 16 mm, and 7 mm respectively. The MIC value of each test bacterium was different depending on the type of bacteria where the growth of Staphilococcus aureus was inhibited at a concentration of 6%, Eschericihia coli 3%, and Pseudomonas aeruginosa 12%. Keywords: Secondary metabolites, Cromolaena odorata, Antibacterial, Chromatography. PermaLink | Plain Format | Corresponding Author (Endang Sulistyarini Gultom)

10	Biology	ABS-331
Feed Conditions Preference for type Ant (Oecophyla smaragdina) In Morphometric Swiftlet (Aerodramus fuciphagus) Sunu Kuntjoro, Fida Rachmadiarti, Herlina Fitrihidajati UNESA Abstract Swiftlet bird is a type of bird that produces a product in the form of a nest made from saliva. The reduced level of endurance of swiftlet birds was due to suboptimal feeding. The aim of this study was to determine the morphometric differences in swiftlets with different feed conditions of the ant species (Oecophyla smaragdina) to the weight gain, length and length of the tail and wings of the swiftlet bird (Aerodramus fuchipaghus) and to find the most optimal feed conditions of the ant species (Oecophyla smaragdina) for the growth of swiftlet birds (Aerodramus fuchipaghus). This type of research was experimental in the Swiftlet bird Sidayu home, Gresik, East Java. The data obtained were then analyzed descriptively. The results obtained morphometric differences in the administration of different types of ant. Furthermore, the optimal growth of swiftlet was in ant species in the form of white ants with an average growth of 11.18 gr (weight), 6.32 cm (wing length), 2.88 cm (tail length) and 7.14 cm ( body length). Keywords: swiftlet bird (Aerodramus fuchipaghus), ant (Oecophyla smaragdina) PermaLink | Plain Format | Corresponding Author (Sunu Kuntjoro)

11	Biology	ABS-332
STUDY OF SOYA ADDITION IN TRIS BASE EXTENDER ON THE QUALITY OF SENDURO GOATS SPERMATOZOA AND MEMBRANE INTEGRITY ON STORAGE Nur Ducha, Widowati Budijastuti Unesa Abstract Soya is an ingredient that is often added to semen extender as a component of macromolecules. This study aims to examine the effect of adding soy in the Tris base diluent on the quality and integrity of the Senduro goat spermatozoa membrane. Fresh semen was collected from the Senduro Goat using an artificial vagina, then observing the motility, viability, and integrity of the spermatozoa membrane. Fresh semen was diluted in tris base extender with different concentrations of soy, ie 0% (as a control group), 1%, 2%, and 3%. Stability of spermatozoa was analyzed based on the quality of spermatozoa, namely motility and viability, and membrane intebrity. Observation of motility and viability is done every day. Spermatozoa motility was observed under a microscope at 200 X magnification, by two people. Viability of spermatozoa was observed using negosin eosin staining. Spermatozoa membrane integrity was observed using the HOST method. The results showed the greatest motility, viability and integrity of the spermatozoa membrane at the beginning of storage were found in the tris + soya diluent of 2%. But at the end of storage, ie on the second day after storage, the quality of spermatozoa in the control group and the treatment group (2% and 3%) did not differ only. Based on the results of the study it can be concluded that the use of soy in the tris base diluent has not been able to provide optimal protection in Spermatozoa Senduro Goat druing storage at temperature of 4-5⁰C. Keywords: spermatozoa od Goat Senduro, soya, tris base extender, temperature of 4-5⁰C PermaLink | Plain Format | Corresponding Author (Nur Ducha)

12	Biology	ABS-84
Analysis Quality of Bioplastic From Kombucha Celullulose for Fruit Packaging Material as Biodegradable Plastic Anggita Rahmi Hafsari, Nia Nuraeni, Ade Kartika Apriliani Departement Biology, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung. Jl. AH. Nasution No 105 Bandung, 40614 Abstract The purpose of this research was to obtain information about the best quality of bioplastic fruit packed using kombucha cellulose which grow in green tea media (Camellia sinensis L.). This research was carried out with a trial method of Completely Randomized Design (CRD) 5 arrangements with 4 replications and a total sample of 20. By administering 1%, 2%, 3%, 4% and 5% glycerol. The statistical analysis used is Analysis of One-way Variance (ANOVA). The characteristics of Bioplastic packaging from kombucha cellulose with the approval of glycerol concentrations of 1%-5% (v / v) produced a combined average mean value of 0.015 mm, 0.02 mm , 0.025 mm, 0.027 mm and 0.045 mm. The tensile strength values obtained were 7.22 N / mm2, 3.27 N / mm2, 2.17 N / mm2, 1.80 N / mm2 and 2.15 N / mm2. The highest percent value was 73%,. While the highest solubility values were 50%. Application of kombucha cellulose as a bioplastic packaging in tomato fruit is acceptable as an alternative packaging fruit biodegradable material for fruit. Keywords: Bioplastic Packaging Fruit, Kombucha Celullose, Camellia sinensis L.Biodegradable PermaLink | Plain Format | Corresponding Author (Anggita Hafsari)

13	Biology	ABS-85
Pleurotus flabellatus Growth On Various Seeds Media and its Potential as Antitumor Anggita Rahmi Hafsari, Vina Departement Biologi, Faculty of Science dan Technology, UIN Sunan Gunung Djati Bandung, Jl. A.H. Nasution No.105 Bandung. Cipadung, Cibiru, Kota Bandung, Jawa Barat 40614, Indonesia Abstract The purpose of this research is to determine the the best media form different seeds media for the growth of pink oyster mushroom mycelium (Pleurotus flabellatus) and the secondary metabolite composition as anti-tumor drugs. The method used was the experimental research method, one factorial Completely Randomized Design with four treatments on different seeds sources in solid and liquid media. To determine the best media we compare the weight of biomass and the secondary metabolites composition. The results showed that the growth of corn media diameter had the fastest growth rate of 86% and 82% control, 61% significantly different in soybeans and 63% in green beans. Biomass results show that the most effective media to obtain biomass is that the green beans have a weight of 26 mg and corn 25.6 mg, while potatoes are 14.7 mg and soybeans 5.9 mg. All carbohydrates media can be used as a medium for the growth of pink oyster mushroom mycelium. The secondary metabolites detected were alkaloids and saponins, while steroids and tannins were not detected in all samples, so pink oyster mushrooms have the potential to have antitumor content. Keywords: Pink Oyster Mushroom, Pleurotus flabellatus, Metabolites Secondary, Antitumor PermaLink | Plain Format | Corresponding Author (Anggita Hafsari)

14	Biology	ABS-176
ISOLATION AND IDENTIFICATION OF HOUSEKEEPING GENE IN Anguilla bicolor Diah Kusumawaty*1, Tina Yulianti1, Hernawaty1 Prodi Biologi Departemen Pendidikan Biologi FPMIPA Universitas Pendidikan Indonesia, Jalan Dr. Setiabudi no 229 Bandung 40154 Indonesia *diah.kusumawaty[at]upi.edu Abstract Housekeeping gene are effective genes needed in cell maintenance and often used as internal controls in gene expression normalization because of their stable nature. However, information from the housekeeping gene especially on Anguilla bicolor is unknown. In relation that, this study aims to isolate and sequencing housekeeping of Anguilla bicolor by designing degenerate primer. 18s ribosomal proteins (18S rRNA), Beta Actin (ACTB), Elongation Factor 1-Alpha (ef1a) and Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). The degenerate primer consists of two primer sets (outer and inner) were designed by: (1) Collection fish sequences of Actinopterygii class; (2) Aligment sequences; (3) Uploading sequences to the Primaclade web; (4) Selection of primary pairs. The selected primers were then used to amplify housekeeping gene Anguilla bicolor using the Nested PCR method. DNA of Anguilla bicolor was obtained from DNA isolation using the Sambrook protocol. The result of housekeeping gene amplification was then sequenced directly to determine the nucleotide sequences of the gene sequences. Next, contig and BLAST analysis are performed on the gene sequences obtained to ensure that the sequences match the target genes. The results of BLAST analysis showed that the three of four genes sequences were corresponding to the target gene, those are ribosome protein 18s (18S rRNA), Beta Actin (ACTB) and Elongation Factor 1-Alpha (ef1a). The general grouping of dendrograms showed that the three gene sequences were in groups with gene target. The conclusion of this study is the discovery of three specific sequences which can be used as a source for designing specific primers in subsequent studies. Keywords: Anguilla bicolor, Housekeeping gene, Degenerate Primer, Nested PCR, Specific Primer. PermaLink | Plain Format | Corresponding Author (Diah Kusumawaty)

15	Biology	ABS-218
Capability of Black Soldier Fly, Hermetia illuncens, to Consume Some Weeds in Vegetable Field Yayan Sanjaya, Suhara, M. Nurjhani, M halimah UPI Abstract Capablity of Hermetia illucens to consume some weeds were conducted. This is an alternative way to reduce weeds in vegetable fields. A descriptive research was conducted by investigating role of BSF on some weeds, ki rinyuh (Chromolaena odorata), Babadotan (Ageratum conyzoides), rumput gajah (Pennisetum purpreum). Each was given BSF larvae with 150 g, 200 gr and 250 gr of weight on 500 gr weed, The result showed that BSF larvae at 50 mg of weight convert weed between 80- 100%, on 100 mg convert 75 % – 97 %, on 150 mg can convert 80 % -100 %. The mortality of BSF larvae on Chromolaena odorata was 90 %, Ageratum conyzoides was 75 %, Pennisetum purpreum was 40%. This showed BSF larvae can be convert weed into BSFlarvae biomass. Keywords: Hermetia illucens, weeds, weight, bioconvesion PermaLink | Plain Format | Corresponding Author (Yayan Sanjaya)

16	Biology	ABS-241
MOLECULAR SCREENING OF AUXILIARY ENZYME IN FUNGUS AS AGENTS IN LIGNOCELLULOLYTIC TRANSFORMATION MATERIALS Emma Mauren Moko(a*), Jantje Ngangi(a), Dino Rahardiyan(b); a Biology Department, Mathematics and Natural Sciences Faculty, Manado State University, Tondano, 95619, Indonesia, emmamoko@unima.ac.id; b Agribusiness Department, Faculty of Agricultural, De La Salle Catholic University, Manado, 95000, Indonesia Universitas Negeri Manado Abstract The abundant supply chain of lignocellulosic waste materials renders this biomass material as a potential source biomaterial for advance materials through various tranformation processes as such biological processes. Lignocellulosic biomass maybe transformed to advance biomaterials with the help of enzymes from extracelluler metabolics of microorganisms such as bacteria and fungi with capacities to hydrolyze lignocellulosic biomass to their smallest fractions. The role of auxiliary enzymes is an important phase in the efficiency of the hydrolyzation and degradation process of various large cellulolytic biomass. This research explores potential auxiliary enzymes in the form of Lytic Polysaccharide Mono-Oxygenases (LPMO) from fungi that are found in symbiotes of lycenes. These lycenes are endemic to trees growing in North Sulawesi in areas of Tomohon, Minahasa and North Minahasa. Molecular screening was conducted from identified fungi isolates, and based on partial genetical analysis on the locus of Internal Transcribed Spacer (ITS) of the fungi’s ribosomal DNA. Screening results discovered of fungi isolates identified as Trichoderma koningiopsis isolate HZA8 and isolate HZA6, Penicillium sumatraense strain CBS 127365 and strain CBS 130380, Trichoderma hamatum isolate PAN12-45 and isolate PAN12-05, Aspergillus aculeatus strain A1.9 18S, Aspergillus aculeatus isolate XSD-74, Trichoderma reesei strain S2606 and isolate 5A14. Keywords: screening, auxiliary enzyms, fungus, lignocellulolytic PermaLink | Plain Format | Corresponding Author (Emma Mauren Moko)

17	Biology	ABS-244
INITIAL STUDY ON THE NATURAL HABITAT, GROWTH ENVIRONMENT AND MORPHOLOGICAL CHARACTERISTICS OF DALUGHA (Cyrtosperma merkusii) AS A POTENTIAL STAPLE FOOD CROP Dino Rahardiyan, Jantje Ngangi, Emma Mauren Moko, Aser Yalindua, Meity Neltje Tanor, Sukmarayu Pieter Gedoan a)Dino Rahardiyan, b)Jantje Ngangi, c)Emma Mauren Moko, d)Aser Yalindua, e)Meity Neltje Tanor, f)Sukmarayu Pieter Gedoan a)Agribusiness Department, Faculty of Agricultural, De La Salle Catholic University, Manado, 95000, Indonesia, drahardiyan[at]unikadelasalle.ac.id b,c,d,e,f) Biology Department, Mathematics and Natural Sciences Faculty, Manado State University Abstract Locally known as Dalugha (Cyrtosperma merkusii) or namely Giant Swamp Taro (GST) is an endemic underutilized foodcrop of North Sulawesi, that is important amongst the surrounding island dwellers of Sangihe, Talaud and other surrounding islands. In accordance to the Indonesian government’s policy in regards with food security and resilience. The purpose of this research is to record the growth of GST in its natural habitat and its morphological characteristics. Initial surveys within this study indicated that the natural habitat of GST is widely spread in several island among the regencies of Siau and Tagulandang, Sangihe, and Talaud to the farthest island of Miangas bordering North Sulawesi and Southern Philippines. On the mainland of North Sulawesi GST is also found in brackish swamps nearing the shorelines of Tiwoho village, in the North Minahasa Regency. Morphologically GST that was found in these areas were measured and identified while he characteristics of the soil in which is the natural habitat of GST were also analyzed for their chemical compositions. Keywords: natural habitat, growth environment, morphological characteristics, Cyrtosperma merkusii, staple food crop PermaLink | Plain Format | Corresponding Author (Dino Rahardiyan)

18	Biology	ABS-255
IDENTIFICATION AND CHARACTERIZATION OF POTENTIAL CELLULOLYTIC BACTERIA FROM THE DIGESTIVE TRACTS OF NORTH SULAWESI TERMITES BASED ON 16S RNA GENE SEQUENCE a*)Jantje Ngangi, a)Emma Mauren Moko, b)Dino Rahardiyan a)Biology Department, Mathematics and Natural Sciences Faculty, Manado State University, Tondano, 95619 Indonesia, jantjengangi[at]unima.ac.id b)Agribusiness Department, Faculty of Agricultural, De La Salle Catholic University, Manado, 95000, Indonesia Abstract Agricultural waste in the form of lignocellulose biomass are considered to be the largest promising carbon source in the nature for transformation to its base components such as simple sugars that can be further converted to bioethanol through cellulolytic enzyme activities. Cellulolytic enzymes can be found in microorganisms such as in the digestion tracts of termites. The microbiological biodiversity in the digestive tract of termites is suspected to produce cellulolytic enzymes that would degrade lignocellulose and may have the potentials to result in bioethanol. The purpose of this research is to identify potential cellulolytic enzym producting bacteria from the digestion tract of termites from various locations of North Sulawesi. Bacteria that was screened was then isolated with PrestoTM Mini gDNA Bacteria Kit Protocol, Geneaid then continued on to 16S RNA Gene Sequencing. Several bacteria isolates were succesfully isolated from the termites digestive tract (genus Odontotermes sp and Nasutitermes sp). Molecular identification with 16S rRNA sequences indicated that isolate B1 had 97% similarity with Bacillus cereus strain L-7, isolate B2 had a similarity of 97% with Stenotrophomonas maltophillia Strain W8-8, while the two isolate isolated from Nasutitermes sp, had a degree of similarity of 99% to E. faecalis. Keywords: cellulolytic bacteria, digestive tracts, North Sulawesi, termites, 16s RNA PermaLink | Plain Format | Corresponding Author (Jantje Ngangi)

19	Biology Education	ABS-272
Effectiveness of Learning Material by ICT-Based Guided Inquiry Model to Train Critical Thinking Skill and Science Literacy of Student in SMPN 1 Atap Kenduruan Sifak Indana (a*), Rudiana Agustini(a), Yuni Sri Rahayu(a) a) Science Education Study Program, Master Program, Universitas Negeri Surabaya Abstract The purpose of this study was describing effectiveness rate of learning material by ICT-based Guided Inquiry Model in training critical thinking skill and science literacy junior high school student. Before being tested to junior high school students, the developed learning material was assessed by expert for the validity rate. It was known that the developed learning material was very valid. Then, the learning material was tested on 30 seventh-grade students of SMPN SATAP Kenduruan, Tuban, East Java, batch of 2017/2018. This data was obtained by critical thinking and science literacy test method. The result of this study showed that the average of pretest was 39.9 point (very less critical) and the average of posttest was 75.5 point (critical) by N-gain score was 0.7, categorized as high score. That result could explain us that critical thinking skill of students in SMPN Satap Kenduruan was increased, from very less critical to critical. So, it can be concluded that learning material by ICT-based guided inquiry model was effective for training critical thinking skill and junior high school students’ science literacy. Keywords: learning material; Guided inquiry model; ICT; critical thinking; science literacy; junior high school PermaLink | Plain Format | Corresponding Author (Sifak Indana)

20	Biology Education	ABS-273
The application of MBVI to identify students’ biological - mathematical attitude in quantitative literacy- based ecosystem instruction E Nuraeni, S A Soesilawaty, I Permana, S Rahmania UPI Abstract This study aims to obtain information and to analyze the biological - mathematical attitudes of students in quantitative literacy- based ecosystem instruction. The research used a quasi-experimental design with 131 six grade students in two high school.. The Biological - mathematical attitude was obtained with the Math-Biology Values Instrument (MBVI) questionnaire and quantitative literacy was asses with multiple choice questions. The results shows that majority of students feel interested, feel the benefits and feel the importance of mathematical abilities in solving biological problems, and almost all students do not feel difficulties with mathematics in ecosystems instruction. Student very high interpretation and calculation abilities as well as high representation and analysis skills. Keywords: MBVI, biological-mathematical attitude, quantitative literacy, ecosystem PermaLink | Plain Format | Corresponding Author (Eni Nuraeni)

21	Biology Education	ABS-38
Interpreting and validating performance assessment for student’s argumentation skills in inquiry-based scientific reading Riezky Maya Probosari, Sajidan, Suranto, Baskoro Adi Prayitno Sebelas Maret University Abstract This research was conducted to validate and interpret 80-item tests of scientific argumentation skills. The tests tested covered some aspects of argumentation test based on Toulmin Argumentation Pattern (TAP). Fifty three students on their third semester in Biology Education Study Program in Central Java, Indonesia, have participated in this research. The Winsteps software program version 3.73 was used to conduct the Rasch analyses in this research. The results showed that (1) the majority of items displayed match the Rasch model, In the summary of the person response pattern, the infit value and mean square outfit are 1.0 or perfect, infit and z-std outfit are close to the ideal value of 0.0. Person reliability 0.73, means that the consistency of student responses is quite good and items reliability 0.83 means the quality of the items is good. Cronbach alpha value is 0.77, means that the interaction between person and item is good. It also showed the difficulty level of the items given: difficult, medium and easy. It can be said that the instrument can measure students argumentation abilities in every aspect. Keywords: Argumentation, TAP, Rasch model, assessment PermaLink | Plain Format | Corresponding Author (Riezky Probosari)

22	Biology Education	ABS-309
Student Perception Of Biological Learning Activities Based On Information And Communication Technology Deni Cendrianto (a*), Bambang Supriatno (b), and Amprasto (b) a) Student of Biology Study Program, Graduate School, Indonesia Education University, St. Dr. Setiabudi No. 229, Bandung 40154, Indonesia denicendrianto[at]upi.edu b)Department of Biology Education, Indonesia Education University, St. Dr. Setiabudi No. 229, Bandung 40154, Indonesia Abstract This preliminary research aim to give description about students perception towards biology learning activities based on information and communication technology. This research used quantitative survey research method with deep explanation. The data collection used questionnaire and interview. The statements in the questionnaire were open and close statements, which showed positive and negative perception with 4 point Likert scales. Numeric indices of appreciation of biology learning activities based on information and communication technology were determined using questionnaire information from 58 students in tenth grade. The result show that out of 58 respondents, most of the express positive perception towards biology learning activities based on information and communication technology. The students positive perception towards biology learning activities based on information and communication technology activity implies can made them become more active in learning, most students said they didnt like learning that only listened to lectures from teachers, they preferred learning activities that involved them actively in learning and that the use of gadgets in learning made them more active in learning. Based on the survey results (56.9%) students do not feel difficulties in using ICT when learning biology, this is because they are digital natives who are used to using digital equipment. Keywords: students; information and communication technology; biology education PermaLink | Plain Format | Corresponding Author (Deni Cendrianto)

23	Biology Education	ABS-57
Improving Creative Thinking Skill through Project-oriented Read-Answer-Discuss-Explain-and Create (RADEC) Learning Model Septinaningrum1,3*, W Sopandi1,2, Y Gumala1,4, P Anggraeni1,5, A H Rahayu1,5, Tursinawati1,6. 1Program Studi Pendidikan Dasar, Jl. Dr. Setiabudi No. 229 Bandung 40154, Indonesia 2Departemen Pendidikan Kimia, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi No. 229 Bandung 40154, Indonesia 3Jurusan Pendidikan Guru Madrasah Ibtidaiyah, IAIN Tulungagung, Jl. Mayor Sujadi Timur No. 46 Tulungagung 66221, Indonesia 4Departemen Pendidikan Guru Sekolah Dasar, Universitas Bhayangkara Jakarta Raya, Jl. Darmawangsa 1 No 1 Jakarta 12140, Indonesia 5 Prodi Pendidikan Guru Sekolah Dasar, STKIP Sebelas April, Jl. Angkrek No. 19 Sumedang 45323, Indosesia 6 Prodi Pendidikan Guru Sekolah Dasar, Universitas Syah Kuala, Jl. Tgk Hasan Krueng Kalee Banda Aceh 23111, Indonesia Abstract Abstract. This research was aimed at investigating the development of students’ creative thinking skill through project-project oriented Read-Answer-Discuss-Explain-and Create (RADEC) learning model. The background of this research was the low students’ creative thinking skill at the fourth semester of group G department of PGMI, IAIN Tulungagung in Subject of Biology for Elementary Education. Project-oriented RADEC learning model was considered effective to solve the problem. The excellences of this learning model is that its syntactic stage which is certain, easy to understand, and can be implemented based on the students’ characteristics in Indonesia. Moreover, the create stage in this model can integrate knowledge and experience through project so that the students’ stimulating and accommodating ability of the students’ creative ideas. This research applied quantitative research method by using pre experiment one group pre test post test design. The instruments of data collection were test, observation sheet, interview, and field notes. The result of the research showed that there was a significant development of the students’ creative thinking skill such as 40. 1%, flexibility 43. 81%, originality 37%, and elaboration 45. 04%. Therefore, it can be concluded that project-project oriented RADEC learning model can be used to develop the fourth students’ creative thinking skill in Subject of Biology for Elementary School Department of PGMI at IAIN Tulung Agung 2018/2019. Keywords: RADEC learning model, Project-oriented, Creative Thinking Skill PermaLink | Plain Format | Corresponding Author (Septinaningrum -)

24	Biology Education	ABS-321
A comparison of learning the digestion process with comic strips and endoscopic video: the effects on student’s achievement and its contributing factors Ikmanda Nugraha, Lilit Rusyati, Rika Rafikah Agustin International Program on Science Education, Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia Jl. Dr. Setaibudhi No. 229 Bandung 40154 *ikmandanugraha[at]upi.edu Abstract Comic strips and videos are two popular media used in today science education. The objectives of this study were to examine the learning outcomes and to explore the contributing factors that drive students to achieve the learning outcomes. A quasi-experimental design was employed. The participants were university students in the science education major from batch 2015 and 2016. Batch 2015 was assigned as endoscopic video class while batch 2016 was assigned as comic strips class. The results showed that there was no significant difference in students learning outcomes and its highest contributing factor for the learning outcomes was personal motivation followed by the lecturer and the media. We conclude that both media were able to facilitate students learning outcomes but motivating students personal motive was also essential due to the nature of university student as the independent learner. Keywords: learning digestion process; comic strips; endoscopic video PermaLink | Plain Format | Corresponding Author (Ikmanda Nugraha)

25	Biology Education	ABS-325
The Validity and Reliability of Science Virtual Test on Levels of Organization and Cell Transport Topics (SVT-LOCT) to Assess Junior High School Students Science Process Skills Wafa Hanifah (1*), Nuryani Rustaman (2*), Lilit Rusyati (1*) (1*)International Program on Science Education, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi 229, Bandung, 40154, Indonesia (2*) Department of Biology, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi 229, Bandung, 40154, Indonesia Abstract The purpose of this study was to analyze the validity and reliability of science virtual test to assess junior high school students’ science process skills on levels of organization and cell transport topics, SVT-LOCT. The final design of the instrument was administered to 118 students of 7th graders from two schools in Kabupaten Bandung, Indonesia. The three steps to validate SVY-LOCT are content analysis of levels of organization and cell transport topics, expert judgment and readability test, and trial test (small group try out and larger test). SVT-LOCT was judged through education, content, media, and language by experts and readability test was conducted by teachers and students. The validation resulted in 31 test items that represent eight skills of SPS to assess students’ science process skills in levels of organization and cell transport topic. The reliability of SVT-LOCT was considered as high with Cronbach alpha = 0.753 with correlation of XY = 0.65, meaning that the instrument is sufficient to assess students’ science process skills. Keywords: science virtual test, validity, science process skills, levels of organization PermaLink | Plain Format | Corresponding Author (Wafa Hanifah Zulkarnain)

26	Biology Education	ABS-329
Correlation Between Problem Solving Learning Modeling and Preparation of Learning Management Plans for Prospective Biology Teacher Students Rinie Pratiwi Puspitawati, A. Basri Universitas Negeri Surabaya Abstract Research has been conducted to measure the correlation between the management of lectures that apply problem solving with the results of the preparation of the RPP on UNESA Biology teacher candidates. This research is a quasi experiment with the method of "one shoot case study", which is applied to lectures on Plant Anatomy in three classes. The correlated indicators are innovative ideas and reflections generated by students when modeling is done with the material design and learning steps contained in the RPP prepared by students. Data in the form of scores were analyzed by Pearson test. The analysis showed that there was a significant correlation, with a coefficient of 0.663; 0.829 and 0.921 for three different classes. These results indicate that lecturer modeling when managing lectures is effective in teaching the preparation of lesson plans for prospective Biology teachers Keywords: Problem Solving, Learning Modeling PermaLink | Plain Format | Corresponding Author (Rinie Pratiwi Puspitawati)

27	Biology Education	ABS-74
The achievement of 21st century students plant literacy through field trip implementation Sariwulan Diana, Anna Nurul Alfiah, Febby Rizkamariana, Amprasto and Ana Ratna Wulan Departemen Pendidikan Biologi, Universitas Pendidikan Indonesia Abstract A research about the achievement of 21st century students’ plant literacy by field trip implementation has carried out, in which it aims to improve students’ plant literacy. This study used an quasi-experimental method, non-equivalent control group design. This design includes a group of students observed in the pretest phase which is then followed by treatment with field trip implementation and ends with posttest. The same applied to the control group, which only did conventional lab. The 21st century plant literacy is critical thinking and problem solving regarding the concept of plant diversity. Field trip includes determining the aspects to be observed, carrying out observations and collecting data and discussion of results and reporting. Research instruments include a set of plant literacy questions and its rubric, worksheet for field trip students and its rubric, and questionnaire about student response to the implementation of field trip. There was a slight increase (N-gain 0.16) of students’ plant literacy in the experimental class and the control class (N-gain 0.23). Although not significantly different from the control class, but the achievement of 21st century students’ plant literacy have potential to develop through field trip implementation. All students respond positively to the field trip implementation. Keywords: Field trip, 21th century plant literacy, critical thinking and problem solving PermaLink | Plain Format | Corresponding Author (Sariwulan Diana)

28	Biology Education	ABS-83
The effectiveness of modified free inquiry strategies to enhance mastery of molecular biology concepts Evi Suryanti(a,b*), Any Fitriani(a), Sri Redjeki(a), Riandi(a) a) Pendidikan IPA, Sekolah Pascasarjana, Universitas Pendidikan Indonesia, Bandung, Indonesia *evisuryanti97[at]student.upi.edu b) Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Islam Riau, Pekanbaru, Indonesia Abstract This study aims to test the effectiveness of the modified free inquiry strategy in Molecular Biology. The quasi-experimental method used involves one experiment and one control group. Topics covered are DNA Isolation, Electrophoresis, and Polymerase chain reaction (PCR). Multiple choice objective tests have been used as data collection instruments. Statistical differences test shows that no significant difference in mean scores between the two groups on all topics, so it was concluded that modified free inquiry strategy was not effective in Molecular Biology especially on the topic of DNA Isolation, Electrophoresis, and PCR. The implication of this research is that the use of the modified free inquiry strategies can be supplemented with computer-based technologies such as virtual laboratories, and portfolio instruments can be used as alternative assessments to assess students mastery of concepts. Keywords: Free inquiry strategies; DNA isolation; Molecular biology, Mastery of concepts, Effectiveness PermaLink | Plain Format | Corresponding Author (Evi Suryanti)

29	Biology Education	ABS-348
DEVELOPMENT OF PLANT TISSUES ATLAS FOR INSTRUCTIONAL MEDIA AT SENIOR HIGH SCHOOL Ardi, Yudi Agustira Rahmatullah Universitas Negeri Padang Abstract Biology learning requires students to carry out observations in practicum activities to form of concept understanding. Based on the observations in class XI of SMAN 3 and SMAN 7 Padang, it was found that the school had carried out practicum activities, but students had difficulty in practicing, especially plant tissue. They did not find clear observations, less skilled in using microscopes and making plant preparations. In the other hand some microscopes had moldy lenses, and the unavailability of practical guidance media that contained photographs of plant tissue observations slashed manually, so that the plant tissue observations carried out did not go smoothly. This development research using tree stages from the 4D development model, namely definition, design and development. The research subjects were 28 students, one teacher, and two lecturers. The instruments were used interview guides, validity and practicality questionnaires. Based on research conducted, have been produced Plant Tissues Atlas for SMA / MA. The validity test results obtained an average value of 3.73. with very valid criteria from the aspects of the appropriateness of content, language and readability, and presentation. Practicality test results obtained an average value of 3.81 with very practical criteria by teachers and 3.65 with very practical criteria by students in terms of learning activities, ease of use, and benefits of use. Based on these, it can be concluded that the Plant Tissues Atlas developed is very valid and very practical Keywords: Atlas, Plant Tissue, Development Research PermaLink | Plain Format | Corresponding Author (Ardi Ardi)

30	Biology Education	ABS-350
APPLICATION OF CYCLE 5E LEARNING MODEL IN PHOTOSYNTHESIS DISCUSSION TO IMPROVE SKILLS OF SCIENCE PROCESSES AIRMADIDI STATE HIGH SCHOOL STUDENTS Arrijani, Meity N Tanor, Debby J.J. Rayer Biology Study Program, Faculty of Mathematics and Natural Sciences, Manado State University Abstract This research is motivated by the learning conditions in Airmadidi State High School where there are weaknesses including: the learning process is still centered on the teacher as the main source of knowledge, where students do not dare to argue and some students do not pay attention to what is conveyed by the teacher. The objective to be achieved in this study is to improve student learning outcomes in photosynthesis material through the Learning Cycle learning model 5E in class XII Science students at Airmadidi State High School in 2019/2020. This research is a classroom action research conducted at Airmadidi State High School with subjects of class XII science students. The object of research is the Learning Cycle 5E on photosynthesis material. The research process was carried out in two cycles which included five stages, namely planning, implementation, observation, reflection, and evaluation. The data analysis technique used is a qualitative descriptive technique that is by analyzing student development data in the first cycle and second cycle is equipped with an average analysis of cognitive values, affective values, and psychomotor values of students. The results obtained showed that the average student learning outcomes in the first cycle, cognitive domain = 71,67 affective behavioral domain (very good); and psychomotor domain (very interested). Average student learning outcomes in cycle II, cognitive domain = 80.15 (successful) or increased from cycle I; affective domain (very good) or increased from the first cycle; psychomotor domain (very interested) or increase from cycle I. Based on the results of the study, it can be concluded that the Learning Cycle 5E learning model can improve the teaching and learning process, this is supported by an increase in Biology learning outcomes for students of class XII IPA SMA Negeri Airmadidi in the academic year 2019/2020. Keywords: learning outcomes, learning cycle learning model 5e, photosynthesis. PermaLink | Plain Format | Corresponding Author (Arrijani Arrijani)

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