MOLECULAR SCREENING OF AUXILIARY ENZYME IN FUNGUS AS AGENTS IN LIGNOCELLULOLYTIC TRANSFORMATION MATERIALS
Emma Mauren Moko(a*), Jantje Ngangi(a), Dino Rahardiyan(b); a Biology Department, Mathematics and Natural Sciences Faculty, Manado State University, Tondano, 95619, Indonesia, emmamoko@unima.ac.id; b Agribusiness Department, Faculty of Agricultural, De La Salle Catholic University, Manado, 95000, Indonesia

Universitas Negeri Manado

Abstract

The abundant supply chain of lignocellulosic waste materials renders this biomass material as a potential source biomaterial for advance materials through various tranformation processes as such biological processes. Lignocellulosic biomass maybe transformed to advance biomaterials with the help of enzymes from extracelluler metabolics of microorganisms such as bacteria and fungi with capacities to hydrolyze lignocellulosic biomass to their smallest fractions. The role of auxiliary enzymes is an important phase in the efficiency of the hydrolyzation and degradation process of various large cellulolytic biomass. This research explores potential auxiliary enzymes in the form of Lytic Polysaccharide Mono-Oxygenases (LPMO) from fungi that are found in symbiotes of lycenes. These lycenes are endemic to trees growing in North Sulawesi in areas of Tomohon, Minahasa and North Minahasa. Molecular screening was conducted from identified fungi isolates, and based on partial genetical analysis on the locus of Internal Transcribed Spacer (ITS) of the fungi’s ribosomal DNA. Screening results discovered of fungi isolates identified as Trichoderma koningiopsis isolate HZA8 and isolate HZA6, Penicillium sumatraense strain CBS 127365 and strain CBS 130380, Trichoderma hamatum isolate PAN12-45 and isolate PAN12-05, Aspergillus aculeatus strain A1.9 18S, Aspergillus aculeatus isolate XSD-74, Trichoderma reesei strain S2606 and isolate 5A14.

Keywords: screening, auxiliary enzyms, fungus, lignocellulolytic

Topic: Biology