IDENTIFICATION AND CHARACTERIZATION OF POTENTIAL CELLULOLYTIC BACTERIA FROM THE DIGESTIVE TRACTS OF NORTH SULAWESI TERMITES BASED ON 16S RNA GENE SEQUENCE a*)Jantje Ngangi, a)Emma Mauren Moko, b)Dino Rahardiyan
a)Biology Department, Mathematics and Natural Sciences Faculty, Manado State University, Tondano, 95619 Indonesia, jantjengangi[at]unima.ac.id
b)Agribusiness Department, Faculty of Agricultural, De La Salle Catholic University, Manado, 95000, Indonesia
Abstract
Agricultural waste in the form of lignocellulose biomass are considered to be the largest promising carbon source in the nature for transformation to its base components such as simple sugars that can be further converted to bioethanol through cellulolytic enzyme activities. Cellulolytic enzymes can be found in microorganisms such as in the digestion tracts of termites. The microbiological biodiversity in the digestive tract of termites is suspected to produce cellulolytic enzymes that would degrade lignocellulose and may have the potentials to result in bioethanol. The purpose of this research is to identify potential cellulolytic enzym producting bacteria from the digestion tract of termites from various locations of North Sulawesi. Bacteria that was screened was then isolated with PrestoTM Mini gDNA Bacteria Kit Protocol, Geneaid then continued on to 16S RNA Gene Sequencing. Several bacteria isolates were succesfully isolated from the termites digestive tract (genus Odontotermes sp and Nasutitermes sp). Molecular identification with 16S rRNA sequences indicated that isolate B1 had 97% similarity with Bacillus cereus strain L-7, isolate B2 had a similarity of 97% with Stenotrophomonas maltophillia Strain W8-8, while the two isolate isolated from Nasutitermes sp, had a degree of similarity of 99% to E. faecalis.
Keywords: cellulolytic bacteria, digestive tracts, North Sulawesi, termites, 16s RNA
